Journal: Czech Journal of Animal Science
Article Title: Expression of goat RNA binding protein (NANOS2), development and evaluation of anti-NANOS2 IgY antibodies
doi: 10.17221/8129-cjas
Figure Lengend Snippet: Figure 9. Assessment of anti-NANOS2 IgY antibody by immunofluorescent staining of GmGSCsI-SB after over-expression of NANOS2. After NANOS2 over-expression or not, GmGSCs-I-SB was immunostained with different antibodies in cluding Rabbit polyclonal anti-NANOS2 IgG antibody and anti-NANOS2 IgY antibody (red). GFP expression indicated GmGSCs-I-SB was transfected successfully with pNANOS2-IRES2-AcGFP1 and pIRES2-AcGFP1. DNA was labelled with DAPI (blue) (scale bar = 200 µm)
Article Snippet: Antibodies were used with the following dilutions: anti-NANOS2 rabbit polyclonal antibody (1 : 300; Santa Cruz Biotechnology, Inc., Dallas, USA) and anti-NANOS2 IgY (1 : 100; own preparation).
Techniques: Staining, Over Expression, Expressing, Transfection